Loading...
Cet enregistrement a été supprimé. Comme ce document est lié à un enregistrement publié, il est disponible ici à titre de référence uniquement.
Cliquez ici pour consulter l'enregistrement suppriméCe document a été mis à jour. Il ne s’agit pas de la dernière version publiée. Cliquez ici pour voir la dernière version du fichier.
Organisme vivant modifié
(LMO)
L’image ci-dessous identifie l’OVM par son identificateur unique, son nom commercial et un lien vers cette page du Centre d’échange Cliquer dessus pour télécharger une plus grande image sur votre ordinateur Pour du soutien sur comment utiliser, aller dans la page de liens rapides OVM.
Biotech Vac Salmonella
EN
Biotech Vac Salmonella
Non
-
Organisation :Vetanco do Brasil Importação e Exportação Ltda (Vetanco)R. Edgar Marchiori, 255 - Distrito Industrial Benedito Storani, Vinhedo - SP, 13280-000Vinhedo - SP,
, BrésilTéléphone : [49] 9 9119.1020,Télécopieur :E-mail : contato@vetanco.com.br,Site Web : https://www.vetanco.com/br/,
Biotech Vac Salmonella is an oral recombinant veterinary vaccine intended to induce protective immunity against Salmonella caused by different species, including Salmonella enteritidis and Salmonella infantis. To achieve protection against Salmonella serotypes, B. subtilis expresses the TolB protein subunit which acts as the vaccine antigen and induces an immune response that contributes to protection the disease. The final vaccine contains no viable modified bacteria, as the recombinant B. subtilis is inactivated before formulation.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
-
BCH-ORGA-SCBD-103064-6 Organisme Bacillus subtilis (Bacillus, BACIU)Bactéries
Bacillus subtilis 1A6 (ATCC® 39085TM)
EN
pHT01
EN
- Transgénèse par agrobacterium
Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
-
BCH-GENE-SCBD-105183-2 Phosphoserine aminotransferase gene | Salmonella typhimurium (SALTM)Séquence codante de protéines | Changemetns dans la qualité et/ou la teneur en métabolites (Protéines et acides aminés)
Information on the inserted DNA sequence
The modified Bacillus subtilis contains a recombinant pHT01 plasmid carrying a genetic expression cassette for the expression of the TolB antigen from Salmonella enterica subsp. enterica serovar Typhimurium strain LT2 used in the vaccine formulation. The DNA sequence encoding the Salmonella TolB protein was cloned into the pHT01 expression plasmid. The coding sequence was inserted into the BamHI/XbaI multiple cloning site of the expression plasmid.
Expression of the TolB coding sequence is regulated by a LacI/PgroESL regulatory cassette, in which the groESL operon is composed of the groEL chaperonin and groES helper protein from Bacillus subtilis and transcription is controlled by the LacI repressor encoded by the lacI gene and induced by isopropyl β-D-1-thiogalactopyranoside (IPTG). Production of the TolB protein is inactivated by treatment with formalin before being used as a vaccine. The final product is intended to be orally administered to poultry.
The modified Bacillus subtilis contains a recombinant pHT01 plasmid carrying a genetic expression cassette for the expression of the TolB antigen from Salmonella enterica subsp. enterica serovar Typhimurium strain LT2 used in the vaccine formulation. The DNA sequence encoding the Salmonella TolB protein was cloned into the pHT01 expression plasmid. The coding sequence was inserted into the BamHI/XbaI multiple cloning site of the expression plasmid.
Expression of the TolB coding sequence is regulated by a LacI/PgroESL regulatory cassette, in which the groESL operon is composed of the groEL chaperonin and groES helper protein from Bacillus subtilis and transcription is controlled by the LacI repressor encoded by the lacI gene and induced by isopropyl β-D-1-thiogalactopyranoside (IPTG). Production of the TolB protein is inactivated by treatment with formalin before being used as a vaccine. The final product is intended to be orally administered to poultry.
Note
- In order to confirm the construct, the recombinant pHT01 plasmid containing the cloned Salmonella TolB protein gene was first inserted into Escherichia coli TOPO 1 by direct ligation. After confirmation, it was inserted then into Bacillus subtilis 1A6 (ATCC® 39085TM) with the same method.
- The vector pHT01 is made up of 7,956 bp where the DNA sequence encoding the Salmonella TolB protein (tolB gene) is composed of 1,293 bp.
- Polymerase chain reaction, with primers that amplify a 1,346 bp fragment, is used to detect the presence of the recombinant plasmid in Escherichia coli and B. subtilis.
- For selection of positive transformants in Escherichia coli and B. subtilis, pHT01 contains selection markers for antibiotics resistance genes, ampicillin and chloramphenicol.
FR
- Vaccin
FR
- Biotech Vac Salmonella is an inactivated oral subunit vaccine containing a modified derivative produced by Bacillus subtilis. The LM is inactivated prior to incorporation into the final vaccine formulation and is not intended to replicate following administration.
- The transformation event listed is the transformation event provided by the submitter. Should there be another transformation event available or additional information regarding the modification of Bacillus subtilis for the use of vaccine antigen against different Salmonella serotypes, the record will be updated.
- CTNBio Technical Summary: Request for Approval of Commercial Release of the product BIOTECH VAC SALMONELLA [PDF] ( Ministério da Ciência, Tecnologia, Inovações e Comunicações (MCTI); Solicitação de Aprovação de Liberação Comercial; Parecer Técnico; Comissão técnica nacional de biossegurança; National Technical Commission on Biosafety ) [ Portugais ]
- Vetanco: Evaluation of long term immunity and protection against Salmonella spp by orally administrated inactivated vaccine [URL] ( International Poultry Science Forum (IPPE) 2018; BV Science Poster ) [ English ]
- Vetanco and Instituto de Investigación Patobiología: Protection conferred by a subunit Salmonella vaccine against Salmonella infantis in broiler chickens [URL] ( Centro de Investigación en Ciencias Veterinarias y Agronómicas (CICVyA); Instituto Nacional de Tecnología Agropecuaria (INTA); International Poultry Science Forum (IPPE) 2018; BV Science Poster ) [ English ]
